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即将举行的网络研讨会:实现无需离心的流式细胞术样品制备工作流程的标准化

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Videos & Webinars自动流式细胞仪染色流程视频
自动流式细胞仪染色流程视频

本视频演示了AUTO1000完成自动标准细胞染色流程,为流式细胞仪的分析准备样品。该视频设置为40X速度。注意到相对于自动移液的 "速率限制步骤",层流洗涤步骤的执行速度有多快,以及这些孔在洗涤后是多么的清晰。重点包括(分钟:秒)。

  • 添加样品 (0:04)

  • 涡旋(0:30, 0:48, 1:03, 1:19, 2:13, 3:05, 3:28)

  • 加入活体/死体试剂(0:35)和孵化(0:48)

  • 用缓冲液1为层流洗涤灌注(0:55),清洗细胞(1:00)

  • 加入表面抗体(1:07)和孵化(1:21)

  • 在层流洗涤系统上清洗表面抗体(1:29)

  • 去除残留的液体(1:34

  • 加入固定/perm并混合(1:46),然后孵化(2:00)

  • 用缓冲液2为层流洗涤灌注(2:04)并清洗细胞(2:09

  • 加入perm缓冲液(2:18)并孵化(2:26)

  • 加入细胞内抗体并混合(2:31)和孵化(2:54)

  • 取出空管架,换上满管架(2:46

  • 在层流洗涤系统上清洗多余的试剂 (2:59)

  • 最后转移到MTP (3:06)

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